Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes
نویسندگان
چکیده
منابع مشابه
ALOX 15 ( arachidonate 15 - lipoxygenase )
Hugo: ALOX15 Other names: 15-LOX; EC 1.13.11.33; arachidonate omega 6-lipoxygenase; LOG15 Location: 17p13.3 Local order: Genes flanking ALOX15 in centromere to telomere direction on 17p13 are: MYBBP1A 17p13.3 MYB binding protein (P160) 1a, GGT6 17p13.2 homolog of gammaglutamyltransferase 6, LOC124974 17p13.2, thioredoxin 1 pseudogene 4, SMTNL2 17p13.2 smoothelin-like 2, ALOX15 17p13.2 arachidon...
متن کاملPurification of arachidonate 5-lipoxygenase from porcine leukocytes and its reactivity with hydroperoxyeicosatetraenoic acids.
Arachidonate 5-lipoxygenase was purified to near homogeneity from the 105,000 X g supernatant of porcine leukocyte homogenate by immunoaffinity chromatography using a monoclonal anti-5-lipoxygenase antibody. Reaction of the purified enzyme with arachidonic acid produced predominantly 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid with concomitant formation of several more polar compounds in smal...
متن کاملImmunocytochemical localization of arachidonate 15-lipoxygenase in erythrocytes, leukocytes, and airway cells.
In reticulocytes, the enzyme 15-lipoxygenase (15-LO) is believed to contribute to cellular differentiation, and in leukocytes and airway cells 15-LO generates inflammatory mediators. The recent availability of antibodies to 15-LO now allows us to determine which specific cells contain the enzyme, to characterize its subcellular localization, and to determine its expression at the translational ...
متن کاملArachidonate metabolism by human polymorphonuclear leukocytes
Release of arachidonic acid by the membrane phospholipase and metabolism by the 5-lipoxygenase pathway was examined in human polymorphonuclear leukocytes (PMNs). The 5-lipoxygenase pathway is activated when PMNs are given arachidonic acid in ethanol and there is extensive metabolism to 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). This activation event was shown to be altere...
متن کاملQualitative and Quantitative analysis of 3D predicted arachidonate 15-lipoxygenase-B (15-LOX-2) from Homo sapiens
15-Lipoxygenase-2 protein has been reported to play an important role in normal development of prostate, lung, skin, and cornea tissues. It behaves as a suppressor of prostate cancer development by restricting cell cycle progression and implicating a possible protective role against tumor formation. On the basis of the above report, we selected 15-LOX-2 protein to study the structural classific...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16495.x